Job Duties/Responsibilities 75% Research Activities • Perform experiments and analyze experimental samples to study Mycobacterium tuberculosis pathogenesis and cellular immunology. Responsible for generation, recording and analyzing data. Involves the opportunity to work in the biosafety level 3 laboratory. • Assist in the designing of research projects: independently plan and perform complex experiments and analysis, record all experimental protocols and data. Organize and interpret experimental results. • Operate routine lab equipment such as balances, pH meters, centrifuges, thermal cyclers, microscopes. Record results both manually and using image and analysis software. • Maintain training and other regulatory records. • Optimize and standardize gene editing protocols for primary human myeloid cells using CRISPR/Cas9, tissue culture and flow cytometry (processing, staining, acquisition). Other techniques involved will include bacterial and mammalian cell culture, flow cytometry, and fluorescence microscopy. • Perform everyday tasks such as laboratory clean up, replenish supplies as needed. Manage hazardous and chemical waste.
15% Administrative Tasks • Manage lab inventory and day to day orders • Maintain biosafety procedures and administrative records, laboratory paperwork • Assist with writing institutional protocols and manuscripts
10% Supervisory • Oversee the work of undergraduate student interns and rotating graduate students. • • • Assist with experimental design and technical procedures
All required qualifications must be documented on application materials
Required Qualifications • BA/BS in a related science plus at least 1 year of experience and knowledge of, or experience with, the basic techniques or methods required by the position, or an equivalent combination of education and experience to equal 5 years. • Proven computer literacy skills, including Word and Excel • Ability to follow written procedures accurately • Proven organizational and communication skills • Proven ability to multi-task and work in a team environment • Exposure to lab techniques through coursework or summer internships (or equivalent)
Preferred Qualifications • Experience isolating cells from human blood and thawing of cryopreserved cells • Tissue culture experience • Basic knowledge or experience performing flow cytometry • Experience performing molecular biology techniques: e.g. PCR, RT-PCR, sequencing • Basic knowledge of data analysis and programming in R • Prior experience with lab management and organization (shipment, inventories, biosafety inspections, etc) • Capacity for supervisory experience – potential to oversee undergraduate researchers • Targeted experience in molecular biology methods, including E. coli culture, cloning, PCR, gel electrophoresis, and analysis of Sanger DNA sequencing results • Targeted experience in basic immunological methods, such as ELISA and western blotting • Experience with or willingness to learn mammalian tissue culture, flow cytometry, human cell sample processing, and both BSL-2, as well as BSL-3 technical skills including work with pathogenic Mycobacterium tuberculosis
The University of Minnesota, founded in the belief that all people are enriched by understanding, is dedicated to the advancement of learning and the search for truth; to the sharing of this knowledge through education for a diverse community; and to the application of this knowledge to benefit the people of the state, the nation, and the world.